AAV2 Luciferase-eGFP

Catalog #

78462

$545 *

Size: 50 µl x 2

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Adeno-Associated Virus serotype 2 (AAV2) is the best characterized AAV serotype. Nearly all recombinant AAV serotypes utilize the AAV2 inverted terminal repeats (ITRs). AAV2 requires the expression of Heparan Sulfate Proteoglycan (HSPG) on the surface of host cells for binding and internalization. Of nearly all the discovered AAV serotypes, AAV2 has the best transduction efficiency in cell culture and is the best tool for in vitro studies.

These AAV particles constitutively express the firefly (Photinus pyralis) luciferase and eGFP genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript and expression of luciferase and eGFP as two separate proteins.

●

Product Data Gallery

Purified AAV2 Luciferase-eGFP particles.

Transduction of HEK293 cells using AAV2 Luciferase-eGFP particles.

Luciferase activity of HEK293 cells transduced by AAV2 Luciferase-eGFP particles.

Product Info

Storage and Usage

Citations

Species

Wild-type AAV Serotype 2

Supplied As

Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² vector genomes/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.

Formulation

AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.

Storage/Stability

AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle

Applications

Use as a positive control for transduction
Optimize transduction assays and track protein expression over time

Shipping Temperature

-80°C

Notes

Biosafety
Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell’s genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

AAV2 Luciferase-eGFP

PRODUCTS

SERVICES

RESOURCES

COMPANY