AAV2 ZsGreen

Catalog #

78444

$545 *

Size: 50 µl x 2

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Description

Adeno-Associated Virus Serotype 2 (AAV2) is the best characterized AAV serotype. Nearly all recombinant AAV serotypes utilize the AAV2 inverted terminal repeats (ITRs). AAV2 requires the expression of Heparan Sulfate Proteoglycan (HSPG) on the surface of host cells for cell binding and internalization. Of nearly all the discovered AAV serotypes, AAV2 has the best transduction efficiency in cell culture and is the best tool for in vitro studies.

These AAV2 particles constitutively express ZsGreen under a CMV promoter. ZsGreen is a human codon-optimized variant of the green fluorescent protein isolated from reef coral (Zoanthus sp). It has been engineered for higher expression in mammalian cells and is up to four times brighter than enhanced GFP (eGFP). ZsGreen expression and transduction efficiency can easily be verified and optimized by fluorescence microscopy or flow cytometry. ZsGreen has an excitation wavelength of 493 nm and an emission wavelength of 505 nm.

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Product Data Gallery

Purified AAV2 ZsGreen particles.

Transduction of HEK293 cells using AAV2 ZsGreen

Product Info

Storage and Usage

Citations

Species

Wild-type AAV Serotype 2

Supplied As

Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² vector genomes/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.

Purification

The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). The purity will vary with each lot; the exact value is provided with each shipment.

Formulation

AAV2 was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer with 0.01% Pluronic F68.

Storage/Stability

AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle

Applications

Use as a positive control for transduction
Optimize transduction assays and track expression over time

Shipping Temperature

-80°C

Notes

Biosafety
Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell’s genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Regulatory Status

Research Use Only

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AAV2 ZsGreen

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