AAV-DJ Scrambled shRNA Control

Catalog #

78871

$545 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

This AAV-DJ Scrambled shRNA (short-hairpin RNA) Control co-expresses a 29-mer scrambled shRNA sequence under the control of a U6 promoter, and Turbo GFP under the control of a CMV promoter to assess the efficacy of transduction.

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Product Data Gallery

Purified AAV-DJ Scrambled shRNA Control particles.

Transduction of NCI-H358 cells using purified AAV-DJ Scrambled shRNA Control particles.

Product Info

Storage and Usage

Citations

Species

AAV Serotype DJ

Purity

The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). Purity varies with each lot; the exact value will be provided with each shipment.

Supplied As

Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² vector genomes/ml. The titer is determined by qPCR and varies with each lot; the exact value will be provided with each shipment.

Formulation

AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee. 

Background

RNA interference (RNAi) is a highly specific tool for protein knockdown in which a small non-coding RNA binds to the target protein’s mRNA to promote its degradation and reduce protein translation. AAV vector-based expression of RNAi drives prolonged reduction in the level of mRNA targets in live animals. Short-hairpin RNA is one of the most widely used RNA interference methods to inhibit protein translation.

Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to wild-type serotypes.

Storage/Stability

AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle

Applications

Use as a negative control for gene-specific shRNA transduction via AAV vectors.

Shipping Temperature

-80°C

Notes

The AAV-DJ viruses are covered under several patents, including U.S. Patent Nos. 7,588,772, 8,067,014, 8,574,583, and 8,906,387, as well as corresponding foreign patents applications and patent rights. AAV-DJ is used under a license agreement.

Biosafety
Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell’s genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Regulatory Status

Research Use Only

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AAV-DJ Scrambled shRNA Control

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