Recombinant human WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1), full length, encompassing amino acids 2-end. This construct contains an N-terminal FLAG-tag. This protein was affinity purified.
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Synonyms
WW Domain Containing E3 Ubiquitin Protein Ligase 1, WWP1, Atrophin-1 Interacting Protein 5, AIP5, Tiul1
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Product Data Gallery
4-20% SDS-PAGE Coomassie Staining
Inhibition of WWP1 auto-ubiquitination by Methylated Ubiquitin.
*Not all lots have been tested for enzyme activity
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, variable FLAG peptide, 10% glycerol, and 3 mM DTT.
MW
106 kDa
Amino Acids
2-922(end)
Genbank #
NM_007013
UniProt #
Q9H0M0
Tag(s)
N-terminal FLAG-Tag
Background
WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1) is a HECT-type E3 Ub ligase belonging to the NEDD4 family of E3 ligases. WWP1 interacts with and ubiquitinates many substrates, including transcription factor ΔNp63 as well as phosphatase and tensin homolog (PTEN), and regulates the degradation of sodium channels and membrane receptors. Genetic mutations or regulatory defects involving WWP1 are associated with neurological disorders and cancer. Aberrant expression of WWP1 in gastric, prostate, and breast cancer, for instance, is an area of high interest, and therefore, WWP1 represents an excellent candidate for therapeutic targeting in multiple cancer types.
References
1. Li Y. et al., Genome Res. Oncogene 28 (33), 2948-2958 (2009). 2. Chen C. Et al., Int. J. Cancer 124 (12), 2829-2836 (2009).
Storage/Stability
At least 6 months at -80°C.
Assay Conditions
Assay was performed using the WWP1 Intrachain TR-FRET Assay Kit (BPS Bioscience #78554) with WWP1 protein titrated at various concentrations.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.