WNK2, GST-Tag Recombinant

Catalog #

100972

$420 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human WNK2 (WNK lysine deficient protein kinase 2), encompassing amino acids (1-489). This construct contains and N-terminal GST-tag. The recombinant protein was affinity purified.

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Synonyms

Protein Kinase With No Lysine 2, Lysine Deficient Protein Kinase 2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

WNK2 (GST-1-489)

Host Species/Expression System

Sf9 insect cells

Purity

70%

Supplied As

Aqueous buffer solution.

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

100 kDa

Amino Acids

1-489

Specific Activity

2.0 pmol/min/μg

Genbank #

NM_006648

UniProt #

F8W9F9

Tag(s)

N-terminal GST-tag

Background

WNK2 (with no lysine kinase 2) is a cytosolic serine/threonine kinase of the WNK protein family that also include WNK1, 3 and 4. The WNK kinases feature a cysteine residue in place of the conserved lysine residue present in the ATP binding subdomain II of most kinases. These kinases control salt homeostasis and blood pressure. WNK2 is predominantly expressed in the brain, heart muscle, small intestine, and colon. WNK2 is involved in the modulation of growth factor-induced cancer cell proliferation through the MEK1/ERK1/2 pathway. WNK2 expression was found down-regulated epigenetically by promoter methylation in human gliomas.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

10 μl of varying amounts of WNK2 was mixed with 10 μl of 0.5 mg/ml PKA protein substrate. Reaction was initiated by adding 5 μl of 250 μM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only