VSIG-3:VISTA [Biotinylated] Inhibitor Screening Assay Kit

Catalog #

79782

$1,175 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The VSIG-3:VISTA[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of VISTA binding to VSIG-3. This kit comes in a convenient 96-well format, with biotin-labeled VISTA, purified VSIG-3, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled VISTA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, VSIG-3 is coated on a 96-well plate. Next, biotinylated VISTA is incubated with VSIG-3 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against VSIG-3:VISTA [Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

V-set, immunoglobulin domain containing 3, IgSF11, B7-H5

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
79491	VSIG-3, Fc-fusion (IgG1), Avi-Tag	20 µg	-80°C	Avoid freeze/ thaw cycles
71327	B7-H5 Fc fusion, Avi-Tag, Biotin-labeled (VISTA-biotin)	60 µg	-80°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer	50 ml	+4°C
79742	Streptavidin-HRP	10 µl	+4°C
79670	ELISA ECL Substrate A	6 ml	Room Temp
79670	ELISA ECL Substrate B	6 ml	Room Temp
79699	96-well white microplate	1	+4°C

*The initial concentrations of VSIG3 and VISTA-biotin are lot specific and will be on the tubes containing the proteins

UniProt #

VSIG-3: Q5DX21; VISTA: Q9H7M9

Background

V-domain Ig Suppressor of T cell activation (VISTA, or B7-H5) is a type I transmembrane protein that functions as an immune checkpoint. V-Set and Immunoglobulin domain containing 3 (VSIG-3, or IGSF11) is a ligand for VISTA which, when bound on activated T cells, inhibits their proliferation and the production of cytokines and chemokines. VSIG-3 expression is elevated in many cancers, especially glioma and melanoma, so the blockade of the VSIG-3/VISTA pathway represents a promising potential cancer immunotherapy strategy.

References

Wang, J., et al. Immunol. 2019, 156.1: 74-85.
Xu, W., et al. Cell. Mol. Immunol. 2018, 15(5): 438-446.

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Contraindications

Keep final DMSO concentration at or below 1%. Excess DMSO interferes with binding and decreases sensitivity of the assay.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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VSIG-3:VISTA [Biotinylated] Inhibitor Screening Assay Kit

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