UBE2D2, His-Tag Recombinant
Catalog #
79370
$325
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Description
Recombinant Xenopus UBE2D2 (ubiquitin-conjugating enzyme E2 D2), full length. This construct contains an N-terminal His-tag. This protein was affinity purified.
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Synonyms
UBC4, PUBC1, UBC4/5, UBCH5B, E2(17)KB2
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Product Info
Storage and Usage
Citations
Species
Xenopus
Construct
UBE2D2 (His-Full Length) (Xenopus)
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution
Formulation
50 mM Sodium Phosphate, pH 7.0, 300 mM NaCl, 150 mM Imidazole, 0.1 mM PMSF, 0.25 mM DTT, and 25% glycerol
MW
17 kDa
Specific Activity
25 pmol/min/µg
Genbank #
NM_001093036
Background
UBE2D2(UBC4) Protein or ubiquitin-conjugating enzyme E2D2 is a member of the E2 ubiquitin-conjugating enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates E6AP and in vivo inhibition of UBE2D2 can lead to inhibition of E6-stimulated p53 degradation (1). UBE2D2 could conjugate ubiquitin to target proteins in an E6AP-dependent manner.
References
1. Mondal, S., et al., Anal. Biochem. 2016; 510: 41-51.
2. Rolfe, M., et al., Proc. Nat. Acad. Sci. 1995; 92: 3264-3268.