TET2 Homogeneous Assay Kit

Catalog #

78559

$1,750 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TET2 (Tet Methylcytosine dioxygenase 2) Homogeneous Assay Kit is designed to measure the activity of TET2 for screening and profiling applications. The TET2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with enough biotinylated TET2 substrate, TET2 assay buffer, and purified TET2 for 384 reactions.

The key to the TET2 Homogeneous Assay Kit is a highly specific antibody that recognizes the hydroxymethylated substrate, and the increase in Alpha-counts is proportional to the hydroxymethylation of the substrate. First, a sample containing TET2 enzyme is incubated with the reaction mixture. Next, acceptor beads are added, followed by donor beads and reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against TET2? Check out our Demethylase Screening Services.

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Product Data Gallery

TET2 enzyme activity.

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Materials Required But Not Supplied

AlphaLISA® anti-rabbit IgG acceptor beads (Perkin Elmer #AL104C)
AlphaScreen® Streptavidin-conjugated donor beads (Perkin Elmer #6760002S)
Optiplate-384 (Perkin Elmer #6007290)
AlphaScreen® microplate reader

Format

Catalog #	Name	Amount	Storage
50162	TET2, FLAG-Tag, His-Tag (catalytic domain)	3 x 40 µg	-80°C
	Biotin-labeled TET2 Substrate (4 µM)	50 µl	-80°C
	Primary Antibody 27-2	4 µl	-80°C
	4x TET2 Assay Buffer 1	3 ml	-80°C
	4x Detection Buffer 1	2 x 2 ml	-20°C
	Plate sealer	1	Room Temp

Background

TET2 (also known as Tet Methylcytosine dioxygenase 2) belongs to the Ten Eleven Translocation (TET) family of proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 regulates gene expression levels in cancer and immune cells, being a widely recognized tumor suppressor gene. Both homozygous and heterozygous mutations in TET2 are indicative of hematopoietic malignancies, with nonsense and frameshift mutations associated with poor prognosis. In addition, TET2 is one of the most frequently mutated genes in acute myeloid leukemia (AML). Targeting TET2 as therapeutical approach may be complex, as in many cancer types TET2 is inactivated, however it can still be found in some immune cell types and it can be modulated via proteins that regulate TET2 activity.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Screen for molecules that inhibit TET2 activity.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The TET2 Homogenous Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 4% DMSO solution in buffer and using 2.5 µl per well.
Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue, interfere with the assay.
Avoid using potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺).
The presence of the culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

DNA Demethylase

Regulatory Status

Research Use Only