Anti–TET2 monoclonal antibody

Catalog #

25320

$415 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Monoclonal antibody raised in mouse against the a recombinant protein containing the N–terminal 300 amino acids of human TET2 (tet oncogene family member 2).

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Product Info

Storage and Usage

Citations

Isotype

IgG1

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide

Concentration

50 µg/50 µl

Immunogen

recombinant protein

Background

TET2 (UniProt/Swiss–Prot entry Q6N021) is a methylcytosine dioxygenase that catalyzes the conversion of 5–methylcytosine to 5–hydroxymethylcytosine (5–hmC). 5–hmC has been recently discovered in mammalian DNA and is abundant in Purkinje neurons, granule cells, embryonic stem cells, and brain tissue, especially in areas that are associated with higher cognitive function. Although its precise role has still to be shown, recent studies indicate that 5–hmC plays important roles distinct from 5–mC. Early evidence suggests that 5–hmC may represent a new pathway to demethylate DNA involving a repair mechanism converting 5–hmC to cytosine. Mutations in TET2 have been associated with myeloproliferative diseases such as essential thrombocythemia, polycythemia vera and primary myelofibrosis.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Western blot analysis using the monoclonal antibody directed against TET2
Figure 1A. Whole cell extracts from HL60 and NB4 cells (40 µg) were analyzed by Western blot using the antibody against TET2 (Cat. # 25320), diluted 1:2,000 (lane 1 and 2) or 1:1,000 (lane 3 and 4) in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Figure 1B. Western blot on mouse E14 ES cells. The antibody was used at a dilution of 1:1,000.

Immunoprecipitation using the monoclonal antibody directed against TET2
IP was performed on 250 µg HL60 RIPA cell lysate using the antibody against TET2 (Cat. # 25320) (lane 3) or an IgG negative control (lane 2). The samples were analyzed by Western blot analysis as described above. The input sample (25 µg RIPA lysate) was used as a positive control (lane 1).

Applications

WB (1:1000 – 1:1
200)
IP (5 µg/mg of RIPA lysate)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human, mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–TET2 monoclonal antibody

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