Sialidase Fluorogenic Assay Kit

Catalog #

82154

$535 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Sialidase Fluorogenic Assay Kit is designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant Salmonella typhimurium sialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor NCGC00063279 (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control.

The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (λexc/λem = 365 nm/445 nm). The increase in fluorescence at λ=445 nm is directly proportional to Sialidase activity.

Need us to run inhibitor screens or profile your compounds against Sialidase? Check out our Metabolic Enzyme Screening Services.

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Synonyms

Sialidase, N-acylneuraminate glycohydrolase, Neuraminidase (NANase), STNA, nanH, STM0928

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Product Data Gallery

Sialidase activity.

Inhibition of Sialidase activity by NCGC00063279.

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Materials Required But Not Supplied

Fluorescent microtiter plate reader capable of measurement at λex355-375/λem435-455 nm.
Orbital platform.

Format

Catalog #	Name	Amount	Storage
101734	Sialidase, His-Tag*	2 µg	-80°C
	Sialidase Assay Buffer	5 ml	-20°C
	25 mM Sialidase Substrate	10 µl	-20°C
	5x Stop Solution	3 ml	-20°C
	NCGC00063279	300 μg	-20°C
79685	96-well black microplate	1	Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein

UniProt #

P29768

Background

Sialidases are glycosyl hydrolases that release α-2, 3-, α-2, 6- and α-2, 8-glycosidic linkages of terminal sialic acid residues from oligosaccharides, glycoproteins, and glycolipids. The Salmonella typhimurium sialidase NANH has been shown to have a high K_M value (mM range) against polyvalent targets and plays a role in enhancement of NK cell-mediated antibody-dependent cellular cytotoxicity. Salmonella typhimurium sialidase is thought to be an immune-modulatory enzyme, therefore, its regulation may be important for therapy of diseases associated with disorders of the immune system.

References

Gray MA, et al., 2020, Nat. Chem. Biol. 16: 1376-1384

Szijj PA, et al., 2023, Nat. Chem. doi: 10.1038/s41557-023-01280-4.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Sialidase Fluorogenic Assay Kit

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