PDE7A/CRE Reporter - HEK293 Recombinant Cell Line

Catalog #

60413

$9,270 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PDE7A/CRE Reporter-HEK293 line contains the firefly luciferase gene under the control of CRE as well as a constitutive expression construct for human PDE7A (phosphodiesterase 7A, accession number NM_002603), both stably integrated into HEK293 cells. The luciferase expression level from the CRE reporter is used to monitor the activity of PDE7A in the cells.

Interested in screening and profiling inhibitors of PDE7A without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

PDE7, phosphodiesterase 7, CRE reporter, PDE7-A, PDE

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Product Data Gallery

PDE7A overexpression reduces the level of cAMP following forskolin stimulation in PDE7A /CRE-HEK293 cells. This effect is reversed by BRL 50481, a PDE7A inhibitor

BRL50481 inhibition dose response curve in PDE7A /CRE Reporter cells

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293 cells

Species

Human

Supplied As

Each vial contains ~ X 10⁶ cells in 1 ml of 10% DMSO.

Biological Activity

The cell line is validated for the induction of the expression of luciferase reporter by an inhibitor of PDE7A.

Genbank #

NM_002603

UniProt #

Q13946

Mycoplasma Testing

The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.

References

1. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77 (5): 1167-1173.

2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3):110-117.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

•Monitor Thuman PDE7A activity
•Screen for activators or inhibitors of PDE7A

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

PDE

Regulatory Status

Research Use Only