PARP3, GST-tag Recombinant

Catalog #

80503

$490 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PARP3, full length. In this construct the N-terminal end of PARP3 is fused with GST. A short peptide linker placed between GST and PARP3 includes a thrombin site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

This product has been cited 2 times.

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Synonyms

poly (ADP-ribose) polymerase 3, poly(ADP-ribosyl)transferase 3, PARP3, PARP-3

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

PARP3 Activity

PARP3 inhibition curve using a chemiluminescent assay (#80553-1). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

PARP3 (GST-2-540(end))

Host Species/Expression System

Sf9 insect cells

Purity

85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT

88 kDa

Amino Acids

2-540(end)

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Genbank #

NM_001003931

UniProt #

Q9Y6F1

Tag(s)

N-terminal GST-tag

References

Application Reference(s):
Fragment-based ligand design of novel potent inhibitors of tankyrases (2013)

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to the PARP3 Chemiluminescent Assay Kit (BPS Bioscience #80553) with PARP3 titrated 2-fold from 800 ng - 0 ng.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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Datasheets/Protocols

80503 Lot 210429-G

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PARP3, GST-tag Recombinant

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