PAK6 Recombinant

Catalog #

40081

$515 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PAK6 (GenBank Accession No. NM_020168), a.a. 385-680, MW=34.2 kDa, expressed in E. coli.

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Product Data Gallery

PAK6 and BSA

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT

34.2 kDa

Amino Acids

385–680

Specific Activity

2.1 nmoles/min/µg

Genbank #

NM_020168

UniProt #

Q9NQU5

Background

PAK6 is a Ser/Thr protein kinase that is a member of the PAK (p21-activated kinase) family that are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 is highly expressed in the testis and prostate tissues and interacts with the androgen receptor (AR). In response to androgen, PAK6 cotranslocate into the nucleus with AR. PAK6 is weakly expressed in normal prostate epithelium but the expression is increased in primary and metastatic prostate cancer cells and is further increased in tumors that relapse after androgen deprivation therapy.

References

1. Kaur,R., et al., Prostate 68 (14), 1510-1516 (2008).
2. Hosgood,H.D. et al., Carcinogenesis 29 (10), 1938-1943(2008).

Storage/Stability

>6 months at –80°C.

Assay Conditions

Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK5 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40081_Lot110520.pdf

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