PAK1 Recombinant

Catalog #

40072

$515 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PAK1 (GenBank Accession No. NM_002576.4), a.a. 248-545, MW=33.6 kDa, expressed in E. coli.

This product has been cited 3 times.

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Product Data Gallery

Specific Activity

PAK1-KD (µg) BSA (µg)

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT

33.6 kDa

Amino Acids

248–545

Specific Activity

32638 pmoles/min/μg

Genbank #

NM_002576.4

UniProt #

Q13153

Tag(s)

none

Background

PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix.

References

1. Sheehan,K.A., et al., Am. J. Physiol., Cell Physiol. 296 (1), C47-C58 (2009).
2. Reddy,S.D., et al., Cancer Res. 68 (20), 8195- 8200 (2008).

Storage/Stability

>6 months at –80°C.

Assay Conditions

Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK1 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40072_Lot110413.pdf

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