PADI4, FLAG-tag Recombinant

Catalog #

50154

$330 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PADI-4, also known as Peptidyl arginine deiminase, type IV, (GenBank Accession No. NM_012387), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PADI-4, Peptidyl arginine deiminase, type IV, Pad

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PADI4 inhibition curve using a fluorogenic assay (#82111). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide

75 kDa

Amino Acids

1–663(end)

Specific Activity

≥80 pmole/min/µg

Genbank #

NM_012387

UniProt #

Q9UM07

Tag(s)

C-terminal FLAG-tag

References

1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813–23.
2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384–93.
3. Wang Y, et al. (2004). Science 306 (5694): 279–83.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl₂, and 2 mM DTT) containing PADI4 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.
After 30 min incubation at 37°C, the reaction was quenched using 15 µL of 500 mM EDTA (final concentration is 115 mM) followed by measuring the amount of NH3 produced by o-phthalaldehyde (OPT) and dithiothreitol (DTT). Fluorescence was measured at λ_ex413 nm and λ_em476 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Arginine Deiminase

Regulatory Status

Research Use Only