Anti–PADI4 polyclonal antibody

Catalog #

25305

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human PADI4 (Protein–arginine deiminase type–4), using a KLH–conjugated synthetic peptide containing a sequence from the N–terminus of the protein

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Synonyms

PAD, HL–60 PAD, PDI4, PADI5, PDI5, Pad

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Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

Protein arginine deiminases catalyse the conversion of arginine residues in proteins to citrulline. PADI4 (UniProt/Swiss–Prot entry Q9UM07) catalyses the citrullination-deimination of Arg-8 and Arg-17 from histone H3 and Arg-3 from histone H4. This prevents their methylation by CARM1 and HRMT1L2/PRMT1, thereby repressing transcription. PADI4 also citrullinates EP300/P300 at Arg-2142, which favours its interaction with NCOA2/GRIP.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against PADI4 (Cat. No. 25305). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.

Western blot analysis using the antibody directed against PADI4
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the antibody against PADI4 (Cat. No. 25305), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right."

Applications

ELISA (1:1000 – 1:5000)
WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–PADI4 polyclonal antibody

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