NY-ESO-1 TCR (1G4) CD8+ NFAT-Luciferase Reporter Jurkat Cell Line

Catalog #

78769

$14,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

This cell line was generated from T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and NY-ESO-1 TCR (1G4) using lentiviral transduction (CD8a Lentivirus #78648 and NY-ESO-1 -Specific TCR Lentivirus Clone 1G4 #78675). The human TCR clone 1G4 specifically recognizes antigen NY-ESO-1 (New York esophageal squamous cell carcinoma 1).

Figure 1: Illustration of the functional co-culture assay used to validate the NY-ESO-1 TCR (1G4) CD8+ NFAT-Luciferase Reporter Jurkat cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Expression of NY-ESO-1 TCR in NY-ESO-1 TCR (1G4) CD8+ NFAT-Luciferase Reporter Jurkat cells.

T Cell Activation using T2 cells as APC.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2T	BPS Bioscience #78756
Assay Medium 2D	BPS Bioscience #78755
CD8⁺ TCR KO NFAT Luciferase Reporter Jurkat Cell Line	BPS Bioscience #78757
T2 Cell Line	ATCC #CRL-1992
NY-ESO-1 (157-165) Peptide	BPS Bioscience #78758
APC MHC I Dextramer (HLA-A*02:01 SLLMWITQV)	Immudex #WB03247
PE anti-human α/β T Cell Receptor Antibody	Biolegend #306707
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture plate, white, clear bottom

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

NY-ESO-1 (New York esophageal squamous cell carcinoma 1, also known as Cancer/testis antigen 1, or CTAG1B), is an important tumorigenic marker present in malignant cells. Normally expressed only in embryonic testis, this highly immunogenic protein is not usually found in normal tissues, but is re-expressed in multiple myeloma, non-small cell lung carcinoma, and breast and ovarian cancer, making it a promising candidate antigen for cancer immunotherapy. Several NY-ESO-1-directed therapies are being developed including cancer vaccines, anti-NY-ESO-1 adoptive cell therapy, and NY-ESO-1-specific TCR-T cell therapy in combination with checkpoint inhibitors.

CD8 (Cluster of Differentiation 8) is a co-receptor of TCR (T Cell Receptor) and a typical marker of cytotoxic T cells. The TCR protein complex is found on the surface of T cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR results in activation of downstream NFAT (Nuclear factor of Activated T-cells) transcription factors that induce the expression of various cytokines such as interleukin-2 to 4, and TNF-alpha. The use of engineered TCR allows T cells to target specific antigens present in cancer cells via the MHC, expanding the portfolio of antigens that can be targeted in cancer cell therapy.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media by BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, and 1% Penicillin/Streptomycin.

Growth Medium 2T (BPS Bioscience #78756): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 mg/ml of Geneticin, 100 μg/ml Hygromycin B, and 0.25 μg/ml puromycin.

Media Used in Functional Cellular Assay
Assay Medium 2D (BPS Bioscience #78755): RPMI 1640 medium supplemented with 1% FBS.

Applications

Design and optimize co-culture bioassays for NY-ESO-1-specific TCR cell evaluation.
Use as a positive control in experiments evaluating NY-ESO-1 TCR cells.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only