RARβ Luciferase Reporter HEK293 Cell Line
RARβ Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express firefly luciferase, under the control of retinoic acid response elements, and with full length human Retinoic Acid Receptor beta (RARb) (accession #P10826-2). RARβ Luciferase Reporter HEK293 Cell Line is designed for monitoring the activity of RARb. This cell line was functionally validated by stimulation with all-trans retinoic acid.
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Figure 1: Illustration of the mechanism of action of RARγ Luciferase Reporter HEK293 Cell Line. Upon activation with retinoic acid, RAR dimerizes with RXR, and activates RARE and luciferase expression. The level of activation corresponds directly to the luciferase signal.
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Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Media Required for Cell Culture
Name | Ordering Information |
Thaw Medium 6 | BPS Bioscience #60183 |
Growth Medium 6A | BPS Bioscience #79542 |
Materials Used in the Cellular Assay
Name | Ordering Information |
ATRA | Sigma #R2625 |
Assay Medium 6A | BPS Bioscience #82211 |
96-well tissue culture treated white clear-bottom assay plate | Corning #3610 |
ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species.
Retinoic acid receptor (RAR) belongs to the family of nuclear receptors and has three subtypes, RARa, RARb, and RARg. RAR heterodimerizes with retinoic X receptor (RXR) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes. RARβ dysfunction can result in cervical carcinoma. Abnormal promoter DNA hypermethylation has also been linked to cancer.
Petkovich M., et al., 1987 Nature 330(6147): 444-450.
Allenby G., et al., 1993 Proc. Natl. Acad. Sci. USA 90(1): 30-34