RARβ Luciferase Reporter HEK293 Cell Line

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Catalog #
60603
$1,895 *
Size: 2 vials
Qty
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Description

RARβ Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express firefly luciferase, under the control of retinoic acid response elements, and with full length human Retinoic Acid Receptor beta (RARb) (accession #P10826-2). RARβ Luciferase Reporter HEK293 Cell Line is designed for monitoring the activity of RARb. This cell line was functionally validated by stimulation with all-trans retinoic acid.

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Figure 1: Illustration of the mechanism of action of RARγ Luciferase Reporter HEK293 Cell Line. Upon activation with retinoic acid, RAR dimerizes with RXR, and activates RARE and luciferase expression. The level of activation corresponds directly to the luciferase signal.

Interested in screening and profiling inhibitors or activators of Retinoic Acid Receptor beta without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
RAR beta, Retinoic acid receptor beta, RARB
Product Info
Storage and Usage
Citations
Host Cell Line
HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Species
Human
Supplied As
Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Media Required for Cell Culture

Name Ordering Information
Thaw Medium 6 BPS Bioscience #60183
Growth Medium 6A BPS Bioscience #79542

Materials Used in the Cellular Assay

Name Ordering Information
ATRA Sigma #R2625
Assay Medium 6A BPS Bioscience #82211
96-well tissue culture treated white clear-bottom assay plate Corning #3610
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
Luminometer  

 

Biological Activity
This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARβ is confirmed by Western blotting.
Genbank #
P10826-2
UniProt #
P10826-2
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Retinoic acid receptor (RAR) belongs to the family of nuclear receptors and has three subtypes, RARa, RARb, and RARg. RAR heterodimerizes with retinoic X receptor (RXR) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes. RARβ dysfunction can result in cervical carcinoma. Abnormal promoter DNA hypermethylation has also been linked to cancer.

References

Petkovich M., et al., 1987 Nature 330(6147): 444-450.
Allenby G., et al., 1993 Proc. Natl. Acad. Sci. USA 90(1): 30-34