Neutrophil Elastase Inhibitor Screening Assay Kit
The Neutrophil Elastase Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of elastase for screening and profiling applications. The elastase assay kit comes in a convenient 96-well or 384-well format and contains enough purified elastase, fluorochrome-conjugated substrate, and PR-02 buffer for 100 or 400 reactions. This kit also includes Sivelestat as control inhibitor.
Figure 1: Illustration of the assay principle.
PR Substrate 3 is a fluorogenic peptide substrate for neutrophil elastase. In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Proteolysis releases AMC and fluorescence is emitted. The increase in fluorescence is directly proportional to elastase activity.
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- Fluorescent microplate reader capable of reading lexcitation = 360 nm; lemission = 460 nm
- Adjustable micropipettor and sterile tips
96 reactions
| Catalog # | Name | Amount | Storage |
| Native Human Neutrophil Elastase* | > 1 µg | -80°C | |
| PR Substrate 3 | 6.25 µl | -80°C | |
| PR-02 Buffer | 10 ml | -20°C | |
| 10 mM Sivelestat | 20 μl | -80°C | |
| 79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein
384 reactions
| Catalog # | Name | Amount | Storage |
| Native Human Neutrophil Elastase* | 2 x > 1 µg | -80°C | |
| PR Substrate 3 | 2 x 6.25 µl | -80°C | |
| PR-02 Buffer | 2 x 10 ml | -20°C | |
| 10 mM Sivelestat | 20 μl | -80°C | |
| 79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein
Elastase is a serine protease from the chymotrypsin family involved in the degradation of elastin and some viral proteins. Elastase exists as pancreatic and neutrophil elastase, which differ in their specificity. Neutrophil elastase is also known as ELA2 or polymorphonuclear leukocyte elastase and is present in azurophilic granules. Upon neutrophil activation, degranulation occurs and serine proteases, such as elastase, are released into the extracellular space to degrade the matrix proteins of pathogens. Alterations in the levels of elastase, or dysfunction of its endogenous inhibitors, result in alpha-1 antitrypsin deficiency (A1AD), emphysema, cyclic neutropenia, abdominal aortic aneurysms (AAA) and chronic obstructive pulmonary disease (COPD), arthritis and psoriasis. The use of neutrophil elastase inhibitors, such as elafin, and the development of new inhibitors with fewer side effects may benefit patients suffering from elastase-related diseases.
Jakimiuk K, et al., 2021 J Enzyme Inhib Med Chem 36(1): 1016-1028.
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