Modified RIPA Lysis Buffer (Moderate Strong)

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Catalog #

82126

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Description

The Modified RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables the extraction of cytoplasmic and nuclear proteins in mild denaturing conditions. This is an SDS-free buffer optimized and formulated for proteins that might be sensitive to some components of the standard RIPA Buffer. This buffer is compatible with many applications, including ELISA-based assays.

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Synonyms

Cell lysis buffer

Product Info

Storage and Usage

Citations

Formulation

50 mM Tris HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% Triton X-100, and 0.5% sodium deoxycholate

Mycoplasma Testing

Sterile filtered (0.2 μm)

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Modified RIPA Lysis Buffer (Moderate Strong)

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