MNK1 Kinase Assay Kit
The MNK1 Kinase Assay Kit is designed to measure MNK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.
ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number |
Reagent |
Amount |
Storage |
|
| 40078 | MNK1 | 40 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
| 79686 | ATP (500 μM) | 50 µl | -20°C | |
| 40535 | MBP (5 mg/ml) | 100 µl | -20°C | |
| 79696 | 96-well plate, white | 1 | Room Temp |
|
MAPK-interacting serine/threonine-protein kinase 1 (MNK1) is a member of the serine/threonine family that plays a role in mitotic cell cycle progression. Alteration of MNK1 has been shown in various cancers such as breast carcinoma and melanoma. MNK1 phosphorylates eukaryotic translation initiation factor 4E (eIF4E), enhancing phosphorylation of quiescent cells and rendering eIF4E oncogenic.
1. Chrestensen, C.A., et al. 2007. "MNK1 and MNK2 regulation in HER2-overexpressing breast cancer lines." Journal of Biological Chemistry 282(7): 4243-4252.
2. Dreas, A., et al. 2017. "Mitogen-activated protein kinase (MAPK) interacting kinases 1 and 2 (MNK1 and MNK2) as targets for cancer therapy: recent progress in the development of MNK inhibitors." Current Medicinal Chemistry 24(28): 3025-3053.
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