Membrane-Bound TNFα CHO Cell Line

Catalog #

78971

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The Membrane-Bound TNFα CHO Cell Line is a clonal CHO cell line stably expressing human membrane-bound TNFα (tumor necrosis factor alpha) driven by an EF1a promoter. The cells were generated by transduction with Membrane-Bound TNFα (mTNFα) Lentivirus (#78955).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Cell surface expression of TNFα in Membrane-Bound TNFα CHO Cell Line assessed by flow cytometry.

ADCC response of Membrane-Bound TNFα CHO Cell Line co-cultured with ADCC Bioassay Effector Cell V Variant (High Affinity)/NFAT Luciferase Reporter Jurkat Cell Line in the presence of Infliximab Recombinant Monoclonal Antibody, an anti-TNFα antibody.

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3J	BPS Bioscience #79974

Materials Used in Cellular Assay

Name	Ordering Information
Growth Medium 2A	BPS Bioscience #60190
Assay Medium 2A	BPS Bioscience #79621
Thaw Medium 2	BPS Bioscience #60184
ADCC Bioassay Effector Cell V Variant (High Affinity)/NFAT Luciferase Reporter Jurkat Cell Line	BPS Bioscience #60541
Infliximab Recombinant Monoclonal Antibody	ThermoFisher #MA5-41776
Clear-bottom, white 96-well tissue culture-treated plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Tumor necrosis factor (TNF, also known as TNFα) is produced predominantly by activated macrophages and T lymphocytes. It has been identified as a key regulator in inflammatory and immune responses. TNF signaling pathways are triggered by binding to one of two distinct receptors, designated TNFR1 (TNF receptor 1) and TNFR2, which are differentially regulated on various cell types in normal and diseased tissues. TNFα exists in both a trimeric membrane-bound form (mTNFα) and as a soluble protein. TNFα is synthetized in a precursor form, a cell surface type II transmembrane protein, which is cleaved by metalloproteinases such as TACE (TNFα converting enzyme) into a soluble peptide. Soluble TNFα can then bind to its receptors and activate downstream signaling pathways. Transmembrane TNFα can also bind to TNFα receptors and induce cellular responses. For instance, it enhances cytotoxicity of NK cells, while in the liver it can trigger hepatitis. Anti-TNFα antibodies can bind to the mTNFα and trigger antibody-dependent cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC) to destroy the mTNFα-expressing inflammatory cells, being a promising therapy for inflammatory diseases.

References

Wang F., et al., 2017 Mol Med Rep 16: 1021-1030.
Horiuchi T., et al., 2010 Rheumatology 49(7):1215-1228.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3J (BPS Bioscience #79974): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 5 µg/ml of Puromycin.

Media required for Cellular Assay:
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2A (BPS Bioscience #60190): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin, and 200 μg/ml of Hygromycin B.

Assay Medium 2A (BPS Bioscience #79621): RPMI 1640 medium supplemented with 10% low IgG FBS, 1% Penicillin/Streptomycin.

Applications

Use as target cells to measure anti-TNFα antibodies ADCC (antibody dependent cellular cytotoxicity) responses.
Measuring antibody binding affinity to mTNFα.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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Membrane-Bound TNFα CHO Cell Line

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