MEKK2, GST-tag Recombinant

Catalog #

40122

$385 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human MEKK2, also known as MAP3K2 and MEKK2B, GenBank Accession No. NM_006609, full length, N-terminal GST-tag, MW=96.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

MEKK2B, MAP3K3, MAPKKK3, Mitogen-Activated Protein Kinase Kinase Kinase 3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

MEKK2 Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥87%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl , 20% glycerol and 3 mM DTT.

96.5 kDa

Amino Acids

full length

Specific Activity

18 pmol/min/µg

Genbank #

NM_006609

UniProt #

Q9Y2U5

Tag(s)

N-terminal GST-tag

References

1. Blank, J. L. et al. J. Biol. Chem. 271:5361-5368, 1996.
2. Hammaker, D. R. et al. J. Immun. 172:1612-1618, 2004.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo Plus reagents (Promega Corporation, Madison, WI).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40122_Lot120509-G12.pdf

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