MART-1 TCR (DMF4) CD8+ NFAT-Luciferase Reporter Jurkat Cell Line

Catalog #

78772

$14,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

MART-1 TCR (DMF4) CD8⁺NFAT-Luciferase Reporter Jurkat Cell Line is a Jurkat cell line generated from the T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and MART-1-specific TCR (DMF4) using lentiviral transduction (CD8a Lentivirus #78648 and MART-1-Specific TCR Lentivirus Clone DMF4 #78678). The human TCR clone DMF4 specifically recognizes tumor antigen MART-1 (Melanoma-associated antigen recognized by T cells-1, or Melan-A).

Figure 1: Illustration of the functional co-culture assay used to validate the MART-1 TCR (DMF4) CD8⁺ NFAT-Luciferase Reporter Jurkat cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Expression of MART-1 TCR in MART-1 TCR (DMF4) CD8+ NFAT-Luciferase Reporter Jurkat cells.

T Cell Activation using T2 cells as APC.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2T	BPS Bioscience #78756
Assay Medium 2D	BPS Bioscience #78755
CD8⁺ TCR KO NFAT Luciferase Reporter Jurkat Cell Line	BPS Bioscience #78757
T2 Cell Line	ATCC #CRL-1992
MART-1 (26-35) Peptide	BPS Bioscience #78759
MART-1 (26-35, Leu27) peptide	BPS Bioscience #78760
MART-1 (27-35) Peptide	BPS Bioscience #78761
APC MHC I Dextramer (HLA-A*02:01 ELAGIGILTV)	Immudex #WB02162
PE anti-human α/β T Cell Receptor Antibody	Biolegend #306707
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture plate, white, clear bottom

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

MART-1 is a differentiation antigen expressed on the surface of melanocytes. A peptide fragment of the protein is found in MHC complexes that are recognized by CD8⁺ cytotoxic T cells. MART-1 is present in most skin cancers, including melanomas, and is used as a biomarker for diagnostic purposes. Since the expression of the protein is restricted to melanocyte containing tissues (skin and retina), and is not found in other tissues, MART-1 is an attractive target for cancer vaccines and adoptive cell therapy. The MART-1 peptide 26-35 is a fragment commonly associated with MHC and recognized by T cell receptors.

CD8 (Cluster of Differentiation 8) is a co-receptor of TCR and a typical marker of cytotoxic T cells. The TCR protein complex is found on the surface of T cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR results in activation of downstream NFAT (Nuclear factor of Activated T-cells) transcription factors that induce the expression of various cytokines such as interleukin-2 to 4, and TNF-alpha. The use of engineered TCR allows T cells to target specific antigens present in cancer cells, via the MHC, expanding the portfolio of antigens that can be targeted in cancer cell therapy.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media by BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, and 1% Penicillin/Streptomycin.

Growth Medium 2T (BPS Bioscience #78756): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 mg/ml of Geneticin, 100 µg/ml Hygromycin B, and 0.25 µg/ml puromycin.

Media Used in Functional Cellular Assay
Assay Medium 2D (BPS Bioscience #78755): RPMI 1640 medium supplemented with 1% FBS.

Applications

Design and optimize co-culture bioassays for MART-1-specific TCR cell evaluation.
Use as a positive control in experiments evaluating MART-1-specific TCR expressing cells.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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MART-1 TCR (DMF4) CD8+ NFAT-Luciferase Reporter Jurkat Cell Line

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