LAT, His-tag (Human) Recombinant

Catalog #

71188

$320 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human linker for activation of T cells family member 1 (LAT), also known as 36 kDa phospho-tyrosine adapter protein, pp36. GenBank Accession No. NM_001014989* variant 4, a.a. 64-269(end), with N-terminal His-tag, expressed in an E. coli expression system. MW = 24.4 kDa. This protein runs at a higher MW by SDSPAGE due to glycosylation.

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Synonyms

linker for activation of T cells family member 1, phospho-tyrosine adapter protein, pp36

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.

24.4 kDa

Amino Acids

64-269(end)

Genbank #

NM_001014989*

UniProt #

O43561

Tag(s)

N-terminal His-tag

References

1. Zhang, W. et al., Cell. 1998; 92(1): 83- 92.
2. Balagopalan, L. et al., J. Immunol. 2013; 190(8): 3849-53.

Storage/Stability

>6 months at –80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful as a kinase substrate and for screening small molecules and antibodies.

Shipping Temperature

-80°C

Notes

*This corresponds to a.a. 28-233 of variant 2, NM_001014989. The aggregation state of this product has been confirmed to be <10%.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy, Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

71188_Lot140829-G2.pdf

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LAT, His-tag (Human) Recombinant

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