KAT5 (TIP60), GST-Tag Recombinant

Catalog #

79422

$410 *

Size: 10 µg

Qty

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Description

Recombinant human KAT5 (lysine acetyltransferase 5) also known as TIP60, full length and containing the kinase domain. This construct contains an N-terminal GST-tag. This recombinant protein was affinity purified and is kinase active.

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Synonyms

cPLA2 interacting protein ESA1 HTATIP, HTATIP1, PLIP, TIP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

KAT5 (GST-Full length)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

88 kDa

Specific Activity

30 pmol/min/µg

Genbank #

NM_006388

UniProt #

Q92993

Tag(s)

N-terminal GST-tag

Background

KAT5 or K (lysine) acetyltransferase 5 is a signaling protein that belongs to the MYST family of histone acetyl transferases (HATs). KAT5 was originally isolated as an HIV-1 TAT-interactive protein that plays an important role in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins (1). KAT5 protein is a histone acetylase that is involved in DNA repair and apoptosis and plays an important role in signal transduction. KAT5 acts as a cofactor of TAT that is involved in the regulation of HIV gene expression. KAT5 is also a co-regulator of transcription factors that either promote or suppress tumorigenesis (2).

References

1. Kamine, J. et.al. Virology. 1996; 216: 357-366.

2. Gorrini, C. et al. Nature. 2007 448:1063-1067.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Kinase activity was measured using Histone H4 Peptide substrate diluted in water to a final concentration of 1 mg/ml. Increasing amounts of the protein kinase were mixed with 0.25 mg/ml substrate to a 20 μl final volume. The reaction was initiated by addition of 5 μl of [Acetyl 3H]-CoA diluted in Acetyltransferase Assay Buffer, containing 50 mM Tris-HCL, pH 8.0, 0.1 mM EDTA, 5% glycerol and 2 mM fresh DTT for a final reaction volume of 25 µl. After incubating at 30°C for 15 minutes, the reaction was terminated by spotting 20 μl of the mixture onto phosphocellulose paper strips. Once fixed, strips were sequentially washed for at least three times in 50 mM Na₂HPO₄, pH 9.0 with gentle stirring. The blank was determined from a “no substrate” sample. Radioactivity was determined using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Acetyltransferase

Regulatory Status

Research Use Only