GCN5 (KAT2A), FLAG-tag Recombinant

Catalog #

50074

$440 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human GCN5, also known as KAT2A, GCNL2, or PCAF-β, GenBank Accession No. NM_021078, a.a. 362-837 (end), with C-terminal FLAG-tag, MW=55 kDa, expressed in a baculovirus infected Sf9 cell expression system.

This product has been cited 1 time.

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Synonyms

GCN5, KAT2A, GCNL2, PCAF-β, HAT, KAT

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Product Data Gallery

Specific Activity

4-20% SDS-Page Coomassie Staining

GCN5 inhibition curve using a chemiluminescent assay (#50079L). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥47%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol

55 kDa

Amino Acids

362–837 (end)

Specific Activity

≥70 pmole/min/µg

Genbank #

NM_021078

UniProt #

Q92830

Tag(s)

C-terminal FLAG-tag

References

1. Barlev, N.A., et al. (1998). Mol. Cell. Biol. 18 (3): 1349–58.
2. Wang, L., et al. (1997). Mol. Cell. Biol. 17 (1): 519–27.
3. Xu, W., et al. (1998). Mol. Cell. Biol. 18 (10): 5659–69.

Application Reference:
Quantitating the specificity and selectivity of Gcn5-mediated acetylation of histone H3 (2013)

Storage/Stability

>6 months at –80°C

Assay Conditions

Assayed with the BPS Bioscience Fluorogenic GCN5 Assay Kit, Cat. # 50091, per manufacturer's instructions.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Acetyltransferase

Regulatory Status

Research Use Only