JMJD2D Homogeneous Assay Kit

Catalog #

79838

$1,125 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The JMJD2D Homogeneous Assay Kit is designed to measure JMJD2D activity for screening and profiling applications. JMJD2D, also known as KDM4D, is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me³ and H3-K₃₆Me³. The key to the JMJD2D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Scheme 1: Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown below.

Scheme 1: Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts, as shown above.

Need us to run inhibitor screens or profile your compounds against JMJD2D (KDM4D)? Check out our Demethylase Screening Services.

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Synonyms

KDM4D assay kit, Lysine Demethylase 4D kit, Jumonji Domain Containing 2D kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Species

Human

Supplied As

The JMJD2D Homogeneous Assay Kit comes in a convenient AlphaLISA^® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD2D for 384 enzyme reactions.

Materials Required But Not Supplied

AlphaLISA^® anti-mIgG acceptor beads, 5 mg/ml (PerkinElmer #AL105C)
AlphaScreen^® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002S)
Optiplate -384 (PerkinElmer #6007290)
AlphaScreen^® microplate reader
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
50117	JMJD2D (KDM4D)	1 µg	-80°C	Avoid freeze/ thaw cycles!
52140E	Primary antibody 5	20 µl	-80°C
79841	Biotinylated histone H3 peptide substrate	500 rxns	-80°C
52409	4x HDM Assay Buffer 4	3 ml	-80°C
52301	4x Detection buffer	2 ml	-20°C

UniProt #

Q6B0I6

References

1. Whetstine, J.R., et al. Cell 2006; 125(3): 467-481.
2. Fodor, B.D., et al. Genes & Dev. 2006. 20: 1557-1562.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Contraindications

Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

Warnings

This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous. Do not ingest, inhale, get in eyes, on skin, or on clothing. If so, wash thoroughly.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only

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JMJD2D Homogeneous Assay Kit

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