Anti–JMJD2D (KDM4D) polyclonal antibody

Catalog #

25300

$360 *

Size: 100 µl

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Polyclonal antibody raised in rabbit against human JMJD2D, also known as KDM4D (lysine (K)–specific demethylase 4D), using two KLH–conjugated synthetic peptides containing a sequence from the central region of the protein

●

Synonyms

JMJD2D, KDM4D, JHDM3D, FLJ10251

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

KDM4D (UniProtKB/Swiss–Prot entry Q9H3R0), also known as JMJD2D, is a histone demethylase that specifically demethylates Lys–9 of histone H3, thereby playing a central role in histone code. KDM4C does not demethylate histone H3K4, H3K27, H3K36 nor H4K20. It demethylates both di– and trimethylated H3K9, while it has no activity on monomethylated residues.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human KDM4D (cat. No. 25300). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,750.

Applications

ELISA (1:200)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Anti–JMJD2D (KDM4D) polyclonal antibody

PRODUCTS

SERVICES

RESOURCES

COMPANY