IRF Reporter (Luc) – THP-1 Cell line (cGAS-Sting Signaling Pathway)

Catalog #

79858

$1,810 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in the human monocytic cell line THP-1. It contains a firefly luciferase gene driven by multimerized ISRE (Interferon Stimulated Response Element) located upstream of the minimal TATA promoter.

The cGAS-STING pathway acts to detect cytosolic DNA and induce an immune response. Briefly, upon binding DNA, the protein cGAS (cyclic GMP-AMP Synthase) triggers the reaction of GTP and ATP to form cGAMP. cGAMP binds to STING (Stimulator of Interferon Genes) which triggers phosphorylation of IRF3 by serine/threonine kinase TBK1 (TANK-binding kinase 1). IRF3 can then bind to interferon-stimulated responsive elements (ISRE) in the nucleus and leads to IFN-α/β production. The IRF reporter (Luc)-THP-1 cell line is highly responsive to STING and CDS ligands.

Interested in screening and profiling inhibitors or activators of IRF-mediated signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

(Luc)-THP-1, The Interferon Regulatory Factor (IRF), (Luc)-THP-1 cell line

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Product Data Gallery

IFNα/β dose response in IRF reporter (Luc)-THP-1 cells

STING agonist dose response in IRF reporter (Luc)-THP-1 cells

Antagonist dose response in IRF reporter (Luc)-THP-1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1, human monocyte, suspension

Species

Human

Supplied As

Each vial contains ~2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 8	BPS Bioscience #79652
Growth Medium 8A	BPS Bioscience #79653

Materials Required For Cellular Assay

Name	Ordering Information
IFNα	PBL Assay Science # 11100-1
IFNβ	R&D systems #8499-IF-010
STING agonist: 2'3'-cGAMP	Invivogen #tlrl-nacga23
STING agonist: 3'3'-cGAMP	Invivogen #tlrl-nacga
SR-717	Selleckchem #S0853
GSK8612	Selleckchem #S8872
Assay Medium 2B	BPS Bioscience #79619
Growth Medium 8A	BPS Bioscience #79653
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 8 (BPS Bioscience, #79652):
RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin.

Growth Medium 8A (BPS Bioscience, #79653):
RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin plus 1 μg/ml of Puromycin.

Media Required for Functional Cellular Assay
Assay Medium 2B (BPS Bioscience, #79619):
RPMI 1640 medium supplemented with 1% Penicillin/Streptomycin.

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

Screen for cGAS and STING compound activity.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only