CTLA4:B7-1 TR-FRET Assay Kit

Catalog #: 72120
Size: 384 reactions
The CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of CTLA4 binding to B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CTLA4, B7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, Cytotoxic T-lymphocyte-associated protein 4, CD152, HTRF


Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Huxley, P., et al. Chem Biol. 2004 Dec;11(12):1651-8.
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Assay Kit
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