IKCA1 (KCNN4) HEK293 Cell Line

Catalog #
$9,000 *
Size: 2 vials
*US Pricing only. For international pricing, please contact your local distributor.

IKCA1 (KCNN4) HEK293 Cell Line are engineered HEK293 cells expressing human IKCA1, also known as KCNN4 (Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4), Genbank Accession No. NM_002250. This cell line has been validated in a cellular assay using a membrane potential-sensitive fluorescence dye (DiBAC4(3)) and by Western blot for IKCA1 expression.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

IKCA1, KCNN4, Intermediate conductance calcium-activated potassium channel protein 4, IK1, hKCa4, hSK4
Product Info
Storage and Usage
Host Cell Line
HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Supplied As
Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Media Required for Cell Culture

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1B BPS Bioscience #79531
Biological Activity
N-terminal FLAG-tagged human IKCA1 has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. Functional analysis of IKCA1 channel in the stable recombinant HEK293 was accomplished by using the membrane potential–sensitive fluorescence dye DiBAC4(3). When IKCA1-expressed HEK293 cells were stimulated with calcium-specific ionophore ionomycin to raise the intracellular level of Ca2+, IKCA1 channel was activated, leading to hyperpolarization and a decrease in cell fluorescence in the functional fluorescence assay with DiBAC4(3). This decrease of cell fluorescence was blocked by the IKCA1 channel inhibitor, clotrimazole (CLT).
Genbank #
UniProt #
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.


IKCA1, also known as Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4, are part of a potentially heterotetrametric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes, but it is also present in endothelial cells and cardiac fibroblasts. They contribute to vascular smooth muscle cell proliferation, migration and cardiac fibrosis. IKCA1 was found to be present at high levels in breast cancer (BC) cells, and when depleted lead to decreased tumorigenesis.  IKCA1 can be modulated with small molecules, and these may provide a therapeutical opportunity in the treatment of BC and cardiovascular diseases.


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