IGFBP7 (K95R), Avi-His-Tag, Biotin-Labeled Recombinant

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Catalog #

101548

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Description

Recombinant human IGFB7 (insulin-like growth factor binding protein 7), encompassing amino acids 27-282(end). This construct contains mutation of interest (K95R) and a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

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Synonyms

Insulin-like growth factor-binding protein 7, IBP-7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, Prostacyclin-stimulating factor, Tumor-derived adhesion factor (TAF), MAC25, PSF

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD93:IGFBP7 (K95R)-Biotin Binding Assay

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Human

Construct

IGFBP7 (K95R) (27-282(end)-Avi-His)-(Biotin)

Mutation

K95R

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

30 kDa + glycans

Amino Acids

27-282(end)

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_001553.3

UniProt #

Q16270

Tag(s)

C-terminal Avi-His Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

CD93 protein (BPS Bioscience #101543) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated IGFBP7 (K95R) protein (BPS Bioscience #101548), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cytokine

Regulatory Status

Research Use Only

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Datasheets/Protocols

101548 Lot 220831

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IGFBP7 (K95R), Avi-His-Tag, Biotin-Labeled Recombinant

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