Human Insulin Like Growth Factor-I Recombinant

Catalog #

90166-A

$130 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant Human IGF-1 is a disulfide-linked monomeric protein consisting of 71 amino acid residues. IGF-1 migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human IGF-1 mature chain was expressed in E. coli.

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Synonyms

Insulin-Like Growth Factor 1, Somatomedin C, IGF1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS, pH 7.4.

8 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Biological Activity

The ED₅₀ was determined by a cell proliferation assay using FDC-P1 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

UniProt #

P01343

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides. These isoforms are differentially expressed by various tissues. The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% a.a. sequence identity with mouse and rat IGF-I, respectively, and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

References

1. J. Clin. Endocrinol. Metab. October 1, 2009. 94 (10): 3913-3921.
2. Mol. Biol. Cell, Sep 2009, 20: 3810 - 3817.
3. Endocrinology, Sep 2009, 150: 4395 - 4403.

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90166-A Lot 230501

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