HSP90α (N-Terminal) Assay Service
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Target
HSP90α (N-Terminal)
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Description
Screening and/or profiling inhibitor compounds against HSP90α (N-Terminal) ligand binding in a biochemical assay.
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Synonyms
HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Fluorescence Polarization
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Reference Compounds and IC50
Geldanamycin, 0.133 μM
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Assay Principle
The HSP90α Assay is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization.
Target Details
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Protein Family
Heat Shock Proteins / Oxidative Stress
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UniProt
P07900
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Background
Hsp90α is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90α function has been shown to play a role in tumorigenesis and disease progression.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
