FUT4-CD15 CHO Cell Line

Catalog #

78417

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant clonal CHO cell line stably expressing full length human FUT4 (fucosyltransferase 4). Since FUT4 controls the synthesis of antigen CD15, forced expression of FUT4 results in overexpression of CD15 at the cell surface, which was confirmed by flow cytometry. The stable clonal cell line was selected for high levels of CD15 expression compared to the parental CHO-K1 cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Cell surface expression of CD15 in FUT4-CD15 CHO Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3B	BPS Bioscience #79529

UniProt #

P22083

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

FUT4 (fucosyltransferase 4) is an enzyme that attaches fucose to N-acetyllactosamine polysaccharides during the glycosylation process. It is directly involved in the synthesis of the non-sialylated antigen, Lewis x (also known as cluster of differentiation CD15, or SSEA-1), which is attached to O-glycans on the cell surface and plays a role in cell-cell interactions. CD15 is one of the most important blood group antigens and is expressed in several types of immune cells. It functions in leukocyte homing and sperm-egg recognition. It has been implicated in human diseases such as leukocyte adhesion deficiency type 2 and several types of blood cancer, and is believed to play a role in cancer metastasis.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience, #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3B (BPS Bioscience, #79529): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml of Hygromycin B.

Applications

Test antibodies against CD15 or FUT4.
Screen inhibitors or neutralizing antibodies which disrupt the interaction between CD15 and partner proteins/ligands on other cells.

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only