CD33– CHO K1 Recombinant Cell Line (High, Medium and Low Expression)

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Catalog #

79935

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Description

Recombinant CD33-CHO K1 cell line stably expressing the full length human C33/Siglec-3 receptor (GenBank Accession: BC028152.1). Surface expression of CD33 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD33 expression (High(79935-H), Medium(79935-M), Low(799350L)) to mimic different stages of cancer target cells with various CD33 expression levels.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Sialic Acid Binding Ig Like Lectin 3 cell line, Gp67 cell line, Myeloid Cell Surface Antigen CD33 cell line, Siglec-3 cell line, Siglec3 cell line

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Product Data Gallery

Flow cytometry analysis of cell surface expression of CD33 in CHO K1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1

Supplied As

Each vial contains ~ 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Amino Acid Sequence

Human CD33/Siglec-3 (accession number: BC028152.1) was cloned into pCMV3.

Genbank #

BC028152.1

UniProt #

P20138

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza,#LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

Background

belongs to the sialic acid-binding immunoglobulin (Ig)-like family. CD33 can be stimulated by sialic acid residues on glycoproteins or glycolipids, resulting in a signaling cascade that inhibits phagocytosis in cells and suppresses immune cell proliferation. CD33 overexpression is one of the top-ranked risk factors for Alzheimer’s disease, and CD33 is the target of Gemtuzumab ozogamicin, an antibody- chemo drug conjugate for the treatment of patients with acute myeloid leukemia.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3: (BPS Bioscience #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3B (BPS Bioscience #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml Hygromycin B

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only