A20 Fluorogenic Assay Kit

Catalog #

78024

$765 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The A20 Fluorogenic Assay Kit is designed to measure A20 activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

Need us to run inhibitor screens or profile your compounds against A20? Check out our Protease Screening Services or Ubiquitination Screening Services.

This product has been cited 4 times.

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Synonyms

TNFAIP3 kit

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Product Data Gallery

Product Info

Storage and Usage

Citations4

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified A20 protein, Ubiquitin-AMC, and A20 assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading λexc/λem=360 nm/460 nm

Format

Catalog Number	Component	Amount	Storage
80394	A20, FLAG-tag	20 µg	-80°C	Avoid freeze/ thaw cycles!
81150	Ub-AMC (10 μM)	500 µl	-80°C
78025	A20 Assay Buffer	10 ml	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

UniProt #

P21580

Background

A20 or TNFAIP3 is a protein that is integral to controlling NF-kB activation. It was first identified in human umbilical vein endothelial cells, but has now been induced in other cell types. A20 has been implicated in rheumatoid arthritis and other autoinflammatory and autoimmune diseases. It also plays a role in cytokine-induced signaling pathways.

References

1. Kato, M., et al. 2009. "Frequent inactivation of A20 in B-cell lymphomas." Nature 459(7247): 712-716.
2. Boone, D.L., et al. 2004. "The ubiquitin-modifying enzyme A20 is required for termination of Toll-like receptor responses." Nature Immunology 5(10): 1052-1060.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only