Firefly Luciferase Lentivirus (UbC Promoter)

Catalog #

79880

$795 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by a UbC (ubiquitin C) promoter (Figure 1). The ubiquitin C promoter is useful for high-level expression across a broad range of species and tissue types.

Figure 1. Schematic of the lenti-vector used to generate the firefly luciferase lentivirus

Figure 1. Schematic of the lenti-vector used to generate the firefly luciferase lentivirus

This product has been cited 4 times.

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Synonyms

UbC lentivirus

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Product Info

Storage and Usage

Citations4

Supplied As

Two vials (500 μl x 2) of firefly luciferase lentivirus at a titer ≥5 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Biosafety

None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Firefly Luciferase Lentivirus (UbC Promoter)

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