EPHA2, His-tag Recombinant

Catalog #

40190

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human EPHA2 (GenBank Accession No. NM_004431), a.a. 596-end with N-terminal His-tag, MW=47 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

EphA2, Ephrin Type-A Receptor 2, CTPP1, ECK, EPHA-2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.

47 kDa

Amino Acids

596–end

Specific Activity

170 U/mg.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

Genbank #

NM_004431

UniProt #

P29317

Tag(s)

N-terminal His-tag

References

1. Nakada,M., et al., Cancer Res. 64 (9), 3179-3185 (2004).
2. Kataoka,H.,et al., J. Cancer Res. Clin. Oncol. 128 (7), 343-348 (2002).

Storage/Stability

At least 12 months at –80°C

Assay Conditions

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 ?M Na orthovanadate, 0.1 mM ATP, 30 ?g/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 ?g /ml recombinant EPHA2.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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EPHA2, His-tag Recombinant

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