EGR1 Promoter Luciferase Reporter Lentivirus

Catalog #

78664

$860 *

Size: 500 µl x 2

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The Early growth response 1 (EGR1, also known as ZNF268 or NGFi-A) Promoter Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human EGR1 promoter (~1.3 kb, Figure 1). After transduction, activation of the EGR1 promoter in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the EGR1 Promoter Luciferase Reporter Lentivirus.

●

Product Data Gallery

EGR1 Promoter Luciferase Reporter stimulated by EGF and PMA in HepG2 cells

Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer >10⁷ TU/ml. The titer will vary with each lot; the exact value will be provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
Phorbol 12-Myristate 13-Acetate (PMA)	LC Laboratories #P-1680
Recombinant human epidermal growth factor (EGF)	BPS Bioscience #90201
HEK293 growth medium or use Thaw Medium 1	BPS Bioscience #60187
Assay Medium 1D	BPS Bioscience #78745
Polybrene	Millipore #TR-1003-G

Formulation

The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Background

Early growth response factor 1 (EGR1) is a member of the EGR family and an important transcription factor. It contains an activation regulatory region, a repressive regulatory region, and three Cys2-His2 subclass zinc finger structures, all recognizing and binding target genes and regulating their transcription. Widely expressed in many different cell types, EGR1 is important in many physiological processes, including cell proliferation and inflammatory factors. EGR1 is a key molecule implicated in many signaling pathways and can act as a tumor suppressor to help monitor DNA damage. It can also promote tumor cell apoptosis and enhance the anticancer effects of radiotherapy and chemotherapy. Alternatively, increased EGR1 expression in hypoxic microenvironments will maintain tumor cell survival and proliferation. Researching the mechanisms of action of EGR1 can help study potential cancer therapies and new cancer treatment studies.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

Screen for activators or inhibitors of EGR1 promoter activity in transduced cells.
Generation of an EGR1 promoter luciferase reporter stable cell line (puromycin resistant).

Shipping Temperature

-80°C

Notes

To generate an ERG1 promoter luciferase reporter stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of puromycin for antibiotic selection of transduced cells.
The following Lentivirus Reporter Controls are available from BPS Bioscience to meet your experimental needs:
1. Negative Control Luciferase Lentivirus (BPS Bioscience #79578): Ready-to-transduce lentiviral particles expressing firefly luciferase under the control of a minimal promoter. The negative control is important to establish the specificity of any treatment and to determine the background reporter activity.
2. Renilla Luciferase Lentivirus (BPS Bioscience #79565): Ready-to-transduce lentiviral particles expressing Renilla luciferase under the CMV promoter. The RLuc lentivirus can serve as an internal control to overcome sample-to-sample variability when performing dual-luciferase reporter assays.
3. Firefly Luciferase Lentivirus (BPS Bioscience #79692-G, #79692-H, #79692-P): Ready-to-transduce lentiviral particles expressing firefly luciferase under the CMV promoter. It serves as a positive control for transduction optimization studies.

Biosafety
The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Apoptosis

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

EGR1 Promoter Luciferase Reporter Lentivirus

PRODUCTS

SERVICES

RESOURCES

COMPANY