eGFP/Firefly Luciferase OVCAR3 Cell Line

Catalog #

78953

$3,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

eGFP/Firefly Luciferase OVCAR3 Cell Line is the human ovarian cancer cell line OVCAR3 engineered to express firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter. This cell line was generated by transduction with Firefly Luciferase-eGFP Lentivirus (BPS Bioscience #78741).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

eGFP expression and luciferase activity in the eGFP/Firefly Luciferase OVCAR3 Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

OVCAR3, ovarian adenocarcinoma cell line, adhering.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2E	BPS Bioscience #79638

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The OVCAR3 cell line was isolated from a high-grade serous ovarian adenocarcinoma patient refractory to treatment with cisplatin. This cell line is highly tumorigenic and presents an abnormal karyotype. OVCAR3 cells are a suitable model of ovarian cancer for drug resistance studies and hormonal therapy. The presence of eGFP and luciferase allow for easy assay readouts, making this cell line a convenient choice.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2E (BPS Bioscience #79638): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 µg/ml of Puromycin.

Applications

In vitro and in vivo bioluminescence imaging (BLI) and fluorescence imaging.
Use as target cells in CAR-T co-culture killing assays

Shipping Temperature

-80°C

Notes

License Disclosure
Visit for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

NK Cells

Regulatory Status

Research Use Only

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eGFP/Firefly Luciferase OVCAR3 Cell Line

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