Csm6, His-Tag (E. italicus) Recombinant

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Catalog #

101628

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Description

Recombinant Enteroccous italicus EiCsm6 (type III-A CRISPR-associated CARF protein Csm6), full length. This construct contains a C-terminal His-tag. EiCsm6 is an ancillary ribonuclease associated with type III CRISPR-Cas system and contains a CRISPR-associated Rossman fold domain.

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4-20% SDS-PAGE Coomassie Staining

EiCsm6 RNase Activity

Product Info

Storage and Usage

Citations

Species

Enterococcus italicus

Construct

Csm6 (Full Length-His) (E. italicus)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 200 mM imidazole, 1 mM DTT, and 10% glycerol

52 kDa

Genbank #

WP_007208953

Tag(s)

C-terminal His-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Variable amounts of EiCsm6 RNase activity were measured using fluorogenic substrate and read overtime using Tecan plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

CRISPR

Regulatory Status

Research Use Only

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Datasheets/Protocols

101628 lot 230110

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Csm6, His-Tag (E. italicus) Recombinant

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