Chemi-Verse™ SIK2 Kinase Assay Kit

Catalog #

82106

$775 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ SIK2 Kinase Assay Kit is designed to measure SIK2 (salt inducible kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant SIK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Qin-induced kinase, Salt-inducible kinase 2 (SIK-2), Serine/threonine-protein kinase SNF1-like kinase 2, Serine/threonine-protein kinase SIK2, KIAA0781, QIK, SNF1LK2

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Product Data Gallery

Inhibition of SIK2 kinase activity by HG-9-91-01

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	SIK2	2.5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	Amara Peptide (1 mg/ml)	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q9H0K1

Background

SIK (salt-inducible kinases) are serine/threonine kinases of the AMPK (AMP-activated protein kinase) family. They are involved in gluconeogenesis and lipid metabolism inhibition. Dysfunction of SIK has been linked to cancer, due to its association with the TGFβ (transforming growth factor β)-Smad, AKT (protein kinase B), Hippo and NF-κB (nuclear factor kappa-light chain enhancer of activated B cells) pathways. The role of SIK proteins in cancer is complex, with some family members acting as oncogenes while others functioning as suppressors. SIK2 is widely expressed and is a potential oncogene in ovarian, prostate cancer, glioma and diffuse large B-cell lymphoma (DLBCL). It promotes cancer in multiple ways: inhibits cell apoptosis, promotes G1 to S cell phase, modulates glucose metabolism, inhibits autophagy and facilitates EMT (epithelial-mesenchymal transition). It accomplishes these roles by repressing AKT (protein kinase B) phosphorylation, activating PI3K (phosphoinositide 3 kinase) via p85α and linking to the PI3K/AKT/HIF1α pathway. The development of SIK inhibitors is the focus of intense research, but further studies are needed to identify isoform-specific inhibitors targeting SIK2 that can be used in cancer therapy.

References

Sun Z., et al., 2020, Signal Transduction and Targeted Therapy 5:150.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ SIK2 Kinase Assay Kit

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