Chemi-Verse™ NUAK2 Kinase Assay Kit

Catalog #

82232

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ NUAK2 Kinase Assay Kit is designed to measure NUAK2 (NUAK family SF-1 like kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant NUAK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

NUAK family SNF1-like kinase 2, Omphalocele kinase 2, SNF1/AMP kinase-related kinase, SNARK, OMPHK2

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Product Data Gallery

Inhibition of NUAK2 kinase activity by WZ4003.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
101476	NUAK2, GST-Tag*	5 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	CHKtide (1 mg/ml)	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

NM_030952

Background

NUAK2 (NUAK family SNF1-like kinase 2), also known as SNF1/AMP kinase-related kinase (SNARK), is a member of the AMP-activated protein kinase family. It participates in cell-cell interactions and cell motility by regulating the conversion of F-actin to G-actin, contributes to neural development, cell proliferation and apoptosis. Loss of function mutations in NUAK2 can result in anencephaly, due to abnormal Hippo signaling pathway cytoskeletal regulation during neural tube closure. NUAK2 has also been involved in cancer development and progression, and it is overexpressed in gastric and cervical cancer and in glioma. Knockdown of NUAK2 in melanoma and cervical cancer cells, in vitro, inhibits cell proliferation and migration. Further studies and a deeper understanding of NUAK2 role and partners will help identify novel cancer therapy strategies.

References

Bonnard C., et al., 2020 J Exp Med 217(12):e20191561.
Li Y., et al., 2021 Mol Med Rep 24(5):817.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ NUAK2 Kinase Assay Kit

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