Complex of human CDK1 (cyclin-dependent kinase 1) and human cyclin B1 (CCNB1). The proteins were co-expressed in Sf9 insect cells. Recombinant human CDK1, full length encompassing amino acids 2-297(end), contains an N-terminal GST-tag followed by a PreScission Protease Cleavage Site. Recombinant human cyclin B1, full length encompassing amino acids 2-433(end), contains an N-terminal GST-tag followed by a PreScission Protease Cleavage Site. The recombinant proteins were affinity purified.
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Synonyms
Cyclin-dependent kinase 1, CDC and CCNB1, CCNB respectively, CDK-1
40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.
MW
CDK1: 60 kDa; CyclinB1: 75 kDa
Amino Acids
CDK1: 2-297(end); CyclinB1: 2-433(end)
Specific Activity
≥200 pmol/min/μg
Genbank #
CDK1: NM_001786; CyclinB1: NM_031966
UniProt #
CDK1: P06493; CyclinB1: P14635
Tag(s)
N-terminal GST-tag
References
1. Vantieghem, A. et al., J. Biol. Chem. 2002; 277(40): 37718-37731. 2. Rigas, A.C. et al., Oncogene. 2007; 26(55): 7611-7619.
Storage/Stability
At least 6 months at -80°C
Assay Conditions
Assay was done according to CDK1 Assay Kit (BPS Bioscience #79597) with various amounts of CDK1.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.
Applications
Useful for studying enzyme kinetics and screening inhibitors.