CD5 (Human) CRISPR/Cas9 Lentivirus (Integrating)

Catalog #

78119

$795 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily and is associated with the immune system. This protein is a transmembrane glycoprotein found on the surface of thymocytes and may act as a receptor to regulate T-cell proliferation.

The CD5 CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1A promoter, along with 5 sgRNA (single guide RNA) targeting human CD5 driven by a U6 promoter (Figures 1 and 2).

The integrating lentivirus integrates randomly into the cellular genome to express both Cas9 and the sgRNA. Puromycin selection forces high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies may vary, depending on the cell type and the gene of interest.

Figure 1. Schematic of the Lenti-vector used to generate the CD5 CRISPR/Cas9 Lentivirus

Gene Target:	Primer ID:	sgRNA Sequence
CD5	CD5-1	CAGCATCTGTGAAGGCACCG
CD5	CD5-2	TTTCCTGAAGCAATGCTCCA
CD5	CD5-3	AAGCGTCAAAAGTCTGCCAG
CD5	CD5-4	GCTGTAGAACTCCACCACGC
CD5	CD5-5	CGTTCCAACTCGAAGTGCCA

Figure 2. List of sgRNA Sequences in the CD5 CRISPR/Cas9 Lentivirus

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Synonyms

CD5 molecule, T1, LEU1, CD5 protein, CD5 scavenger receptor cysteine-rich superfamily (SRCR), CD5 lentivirus, CD5 CRISPR

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Replication Incompetent HIV

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer ≥1 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

1. Transient knock-down of CD-5 in target cells
2. Generation of stable CD-5 knockout cell pool following puromycin selection and limited dilution

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Biosafety

None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

CRISPR

Regulatory Status

Research Use Only

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CD5 (Human) CRISPR/Cas9 Lentivirus (Integrating)

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