CD244, Fc Fusion (IgG1), Biotin-Labeled (Human) Recombinant

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Catalog #

72306

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Description

Human CD244, Fc fusion protein, also known as Natural Killer Cell Receptor 2B4. GenBank Accession No. NM_016382, a.a. 22-229 expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. MW = 52 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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Synonyms

Natural Killer Cell Receptor 2B4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD48: CD244-Biotin Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

52 kDa+glycans

Amino Acids

22-229

Genbank #

NM_016382

UniProt #

Q9BZW8

Tag(s)

C-terminal Avi-tag™

References

1. Eissmann, P. et al., Blood. 2004; 105: 4722-4729.

2. Watzl, C. et al., Journal of Immunology. 2000; 165(7): 3545-3548.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and for screening small molecules and antibodies.

Shipping Temperature

-80°C

Notes

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

72306_lot_160421.pdf

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CD244, Fc Fusion (IgG1), Biotin-Labeled (Human) Recombinant

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