Search results for: 'parp'

PARP Assays Poly(ADP-ribose) polymerase (PARP) proteins play a crucial role in DNA repair mechanisms within cells. These enzymes are involved in the repair of single-strand DNA breaks through the base excision repair pathway. Inhibition of PARP proteins has emerged as a promising strategy in cancer therapy, particularly in tumors with defective DNA repair mechanisms, such as those with BRCA mutations. When PARP is inhibited, it prevents the repair of single-strand breaks, leading to th...

PARP/PARPTrap™ Screening & Profiling PARP (Poly ADP-Ribose Polymerase) proteins are a large family of 17 members that catalyze the ADP-ribosylation of proteins and DNA. PARPs are part of a network of 150 proteins involved in the DNA damage response, which constantly scan and repair DNA to maintain genome integrity. The PARP proteins are involved in a wide range of biological functions: DNA repair, chromatin remodeling, mitotic spindle assembly, regulati...

Setting a Trap for PARP1 and PARP2 Summary PARP1 and PARP2 operate in DNA damage response (DDR) by sensing single breaks in DNA and by recruiting the repair machinery through poly-ADP ribosylation of histones and other proteins. PARP inhibitors are cytotoxic to cancer cells deficient in repair pathways that are complementary to PARP pathways, and it has been shown that some of these inhibitors act by trapping PARP1/2 onto the DNA, preventing repair and leading to cell death. Thus, DNA tra...

Cellular PARylation: From Inquiry to Insight in Drug Discovery Summary Inhibitors of poly (ADP-ribose) polymerases (PARPs) have been game-changing in cancer therapy, demonstrating the potential efficacy of synthetic lethal drugs targeting complementary pathways within the DNA damage response network. However, the evaluation of poly-ADP-ribosylation in intact cells is difficult, slowing the development of candidate drugs. BPS Bioscience’s LysA™ Universal PARylation Assay Kit quantifies...

Nicotinamide Adenine Dinucleotide Overview NAD+ plays an important role as a cofactor in enzyme catalyzing redox reactions by transferring electrons to/from its reduced form, NADH. It also serves as a substrate for various enzymes such as Sirtuins , Poly ADP-ribose polymerases (PARPs),  and CD38 .  Clinical Importance Recent studies revealed that cellular NAD+ concentration is associated with chronological aging and age-related diseases, suggesting it is important to un...

PARG Screening & Profiling PARG (Poly(ADP-ribose) glycohydrolase) is a protein that plays a crucial role in DNA repair processes, including base excision repair and single-strand break repair. Its function involves removing poly(ADP-ribose) (PAR) from proteins that have been modified by PAR polymerases (PARPs), which enables DNA repair. Dysregulation of PARG activity has been implicated in various diseases, including cancer. Targeting PARG for therapy has ...

NAMPT: Metabolism, Cancer, and Drug Discovery NAMPT & Metabolism The unique metabolic requirements of rapidly reproducing cells are classical targets for cancer treatment.  For example, rapid growth creates a high demand for ATP used in the biosynthesis of DNA and proteins.  The enzymatic cofactors  NAD +  and NADH contribute oxidizing and reducing agents required to generate ATP and regulate the resulting oxidative stress.  This need for high levels of A...

Combining Therapies for Targeting Immune Checkpoints   |  IMMUNE CHECKPOINTS  |           |  KINASES  |                |  PARPS  |              |  HDACS  |               |  CAR T-CELL THERAPY  | Cancer is a balance between the defens...

Screening and Profiling Services Evaluate lead compounds with our biochemical and cell-based screening services to identify specific inhibitors or activators. Save time by letting us screen your small molecules or biologics of interest against our unique assay panels or determine IC 50 values. With our portfolio of > 600 assays available as a service, we’re here to help advance your drug discovery program. Our biochemical-based inhibitor...

DNA Damage Repair DNA damage repair (DDR) processes are critical for maintaining healthy cells, while potentially serving as promising targets for inducing synthetic lethality in cancer cells. DNA damage, resulting in base mismatches, bulky adducts, cross-links, single strand breaks, or double strand breaks, can occur from a variety of exogenous and endogenous mechanisms. In response to such DNA damage, mammalian cells emp...

Bioactive Proteins & Enzymes BPS Bioscience specializes in manufacturing bioactive enzymes and protein complexes, offering over 2000 protein products critical for drug discovery. We feature a large selection of epigenetic and metabolic enzymes, kinases and other cell signaling proteins, as well as cancer-related proteins and their mutants, including KRAS, PARP proteins, and immune checkpoint molecules.  Contact us to learn how our products and services can help you reach your resea...

Custom Biochemical Assay Development BPS Bioscience has the capability to develop robust biochemical assays using our portfolio of assay technologies. Our biochemical assay development services enable you to generate an appropriate assay which is suitable for screening and evaluating your target of interest. Our cross-functional team of scientists can also work closely with you to develop assays for compound library screening as well as for secondary screening support. ...

Apoptosis Screening & Profiling Apoptosis is a form of programmed cell death that results in specific cellular changes including cell shrinkage, membrane blebbing, DNA fragmentation, and nuclear fragmentation. Apoptosis is a normal process that routinely occurs in multicellular organisms to eliminate unwanted cells. In certain disease states, such as cancer, loss of apoptotic pathways results in prolonged tumor cell survival. Therefore, restoring the defec...

BPS Bioscience Assay Kits At the heart of our assay kits are highly active, purified proteins. These quality proteins are then optimized with ancillary reagents and buffers to produce robust, consistent, and simple-to-use assay kits. Our kits make screening for inhibitory compounds or blocking antibodies simple and accurate. We regularly i...

DNA Replication & Repair Screening & Profiling DNA replication and repair are crucial processes for maintaining genetic integrity and ensuring the accurate transmission of genetic information necessary for cell division, growth, and development. Errors in replication can lead to mutations, resulting in genetic disorders and increased susceptibility to diseases like cancer. DNA repair mechanisms play a vital role in identifying and correcting damage to the ...

...

Small Molecule Cancer Therapy Targets For some cancers, small molecules that disrupt, inhibit, or agonize target protein function may be the best therapeutic option. Indeed, many new targeted therapeutics are now approved together with companion diagnostics to identify patient populations most likely to benefit from the new treatment. For some cancers, small molecules that disrupt, inhibit, or agonize target protein function may be the best therapeutic...

Citations   This page contains a list of scientific journal articles that have cited BPS products and services since 2013. We would like to congratulate all our loyal customers on their contributions to the scientific community. Our customers are truly amazing and dedicated scientists and this is evidenced by the outstanding research they are doing.  Search By Product Name or Catalog Number Name Title 10X HAT Assay Buffer (50095...