BRK Kinase Assay Kit
The breast tumor kinase (BRK) Kinase Assay Kit is designed to measure BRK tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent.
- ADP-Glo™ Kinase Assay (Promega #V6930)
- DTT (Dithiothreitol), 1 M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40403 | BRK, GST-Tag* | 10 µg | -80°C |
| 79334 | 5x Kinase Buffer 1 | 1.5 ml | -20°C |
| 79686 | 500 µM ATP | 50 µl | -20°C |
| 40217 | PTK Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) | 50 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
Breast Tumor Kinase (BRK, also known as Tyrosine-protein kinase-6 or PKT6) is a non-receptor protein with tyrosine kinase activity. BRK is involved in epithelial differentiation and apoptosis. It interacts with multiple proteins and is involved in several signaling pathways, like activating STAT3 and STAT5B to promote cell proliferation. It is involved in cancer progression, migration and evasion, namely in breast cancer. Targeting BRK kinase activity via small molecule inhibitors may be a viable therapeutic approach for breast cancer.
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